Before submitting a sample, please arrange for a consultation with PM core staff at (852) 2831 5482 (84) or This email address is being protected from spambots. You need JavaScript enabled to view it..

Sample submission for Proteomics services:

  1. Submit service request through iLab 
  2. Please contact our colleagues in advance (via phone/email) before bringing the samples to the Core
  3. Fill in Proteomics Sample for MALDI-TOF/TOF Submission Form  'OR'
  4. Fill in Proteomics Sample for ESI-LC-MS Submission Form (Orbitrap - Lumos and timsTOF), and attach it under service request in iLab
  5. Bring samples to the Core

 Sample submission for Metabolomics services:

  1. Submit service request through iLab
  2. Please contact our colleagues in advance (via phone/email) before bringing the samples to the Core
  3. Fill in Metabolomics Sample Submission Form and attach it under service request in iLab
  4. Bring samples to the Core

  The sample requirements for various applications on PMCore platforms are given below:

Platform   Applications  Sample type Sample preparation Platform requirement 
MALDI - TOF/TOF

- Protein ID

- Mass Determination

- Gel Plug

- In-solution (undigest or digest -protein/ peptides)

- Spotted peptides on MALDI Plate (384 format)

- Peptides (<5kDa):  Min. 1 pmol/µl

- Proteins: Min. 1 pmol/ µl

- Enzyme digests: Min. 1 pmol/ µl

 

Orbitrap Fusion Lumos (LC-MS/MS)

 

 

timsTOF Pro (LC-MS/MS)

- Protein ID

- Protein Quan (DDA or DIA)

- PTM's-  Phosphorylation site ID & Quan          

Tissue 2mg dry weight 

    50ng- 2ug peptides

Serum / plasma 0.1 ml 
Cell lysate

< 1 million cells or 5-20 ug protein. 

5ug purified protein for Phospho site ID. 

1mg - 2mg protein per condition for Phosphoproteomics

GC-QQQ MS

Targeted Metabolite Quantification Serum or plasma

0.2 ml per sample

Metabolite concentration range: pM - mM
Tissue 20-50 mg per sample
Cell lysate 3-6 million cells per sample
LC-QTRAP MS

Targeted Metabolite Quantification

Serum or plasma 0.2 ml per sample Metabolite concentration range: pM - mM
Tissue 20-50 mg per sample
Cell lysate 3-6 million cells per sample
Kit based Targeted Metabolite Profiling Serum or plasma 10 ul per sample  
Cell lysate 3 million cells per sample
LC-QTOF MS Untargeted Metabolite Profiling Serum or plasma 0.2 ml per sample Metabolite concentration range: nM - mM
Cell lysate 10 million cells per sample
Bio-HPLC

Offline Fractionation

(SCX, SAX, basic RPLC)

Proteins isolated from any samples type   n/a 500ug - 5 mg


Please note
:

  1. Samples will not be accepted without prior consultation. Prioritization will be given to time-sensitive projects, including preliminary data needed for timely grant submissions and additional experiments needed for resubmission of manuscripts.
  2. We also receive ready to inject peptides samples for LC-MS/MS analyses. However, prior to mass spectrometry analysis, samples should be salt, buffer, and detergent free. All other sample solution conditions must be discussed with the staff prior to sample submission. Providing the cleanest samples possible will greatly enhance our ability to provide you with the best mass spectrometry data.