The Proteomics and Metabolomics Core Facility offer following sample preparation services for biological samples such as cells, tissues and body fluids.

  1. Sample homogenization (e.g. Cells and tissues)

  2. Reduction, alkylation, digestion (in-solution or in-gel) and C18 stagetips cleanup

  3. Depletion of High Abundance Proteins (Agilent MARS-14) from serum/plasma 

  4. Isobaric tagging for Relative and Absolute Quantitation (iTRAQ), TMT labeling for Protein ID & Quan.

  5. Multidimensional protein and peptide seperation using UPLC

 

Please note:  

  1. We only provide downstream LC-MS/MS analyses for SILAC labeled protein samples
  2. Based on the project requirements PMcore will decide to choose the sample prep services