The state-of-the-art MS/MS system in PM Core can be tuned and optimized to obtain further structural protein information such as post-translational modifications. These techniques often require specific sample enrichment steps and custom-developed LC-MS/MS methods. 

We provide standard protocol services for the following proteomics applications,

Note: We also undertake customized service request for PTM identification and quantitation via project collaboration

 1. Phosphoproteomics Profiling (TiO2 enrichment)

  • Full service package includes project consultation, sample preparation, TiO2 based enrichment, LC-MS/MS runs and data analysis*
  • We also offer Phospho-enrichment service from the purified protein from Substrate Kinase assays or IP pull down experiments

 Phosphoproteomics Profiling


2. Quantitative Phosphoproteomics: Deep Label-free Approach (TiO2 enrichment)

 

  • Full service package includes project consultation, sample preparation, TiO2 based phosphopeptide enrichment, site localization, Extensive High pH RP-HPLC fractionation, LC-MS/MS runs and data analysis*

Quantitative Phosphoproteomics 


3. Quantitative Phosphoproteomics: Deep Label-free Approach (Sequential IMAC-TiO2 enrichment)

 

  • Full service package includes project consultation, sample preparation, Sequential IMAC and TiO2-enrichment, LC-MS/MS runs and data analysis*

Quantitative Phosphoproteomics 2

Please contact PM core at (852) 2831 5482 or This email address is being protected from spambots. You need JavaScript enabled to view it. to consult whether these experiments can be applied to your research